Plant Tissue Culture: Techniques and Experiments, Fourth Edition, by Sunghun Park PhD, published by Academic Press on February 17, 2021, offers a comprehensive guide to plant culturing, building on a legacy of nearly 30 years of classroom-tested expertise. This essential resource bridges the gap between theory and practice, providing detailed methodology supported by extensive illustrations. The experiments featured are designed to demonstrate major concepts and can be adapted to a wide range of plant materials available year-round. This fully updated edition incorporates the latest advancements, including principles and applications of CRISPR/Cas9 gene editing and RNAi technology within plant cell and tissue cultures. It covers fundamental principles, methods, and techniques applicable to all crop plants – from agronomic and horticultural to forestry crops – for germplasm improvement. Readers will be guided through every step, from initial lab setup, essential supplies, and stock solution preparation to media formulation, explant selection and disinfestation, and the crucial processes of experimental observation and measurement. This edition reflects the most recent updates since the previous publication in 2012, making it an indispensable tool for university students and plant scientists alike.