Molecular Ecology, authored by Joanna R. Freeland, offers a thoroughly updated guide to the increasingly prevalent use of molecular data in ecological studies. The third edition of this essential text reflects breakthroughs achieved in recent years, providing readers with a comprehensive introduction to molecular ecology and its fundamental concepts and principles. Key topics covered include next-generation sequencing, metabarcoding, environmental DNA (eDNA) assays, and epigenetics. Presenting the field’s most recent studies and research developments, this book is an accessible, user-friendly resource for both newcomers and seasoned professionals in the area of molecular ecology. With a focus on local adaptation, dispersal across landscapes, phylogeography, behavioral ecology, and conservation biology, Molecular Ecology is an indispensable reference for anyone interested in understanding the role of genetic science in ecological studies.